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. 2011 Feb 28;286(16):14110–14119. doi: 10.1074/jbc.M110.190702

FIGURE 4.

FIGURE 4.

Modulation of PKA or RSK kinase activity affects wild type K2P3.1 current in HEK293 cells. Cells co-transfected with K2P3.1 and GFP on separate plasmids were grown with or without kinase inhibitor or activator compounds, and then surface currents were measured by whole-cell patch clamp analysis. A, cells were incubated in the presence of 0.4 mm 8-Br-cAMP (PKA activator; △), 50 μm SL0101 (RSK inhibitor; ▾), or DMSO (untreated control; ●). ○, non-transfected cells. B, effect of 8Br-cAMP on wild type hK2P3.1 compared with mutants S393A and S393D in HEK293 cells. Plot shows current at 60 mV with and without 8Br-cAMP. C, cells were incubated in the presence of 1 μm KT5720 (PKA inhibitor; ○), 20 μm PKI (PKA inhibitor; ▾), or DMSO (untreated control; ●). △, GFP alone. D, current at 60 mV, with or without inhibitors or activators from A and C, expressed relative to the untreated control. The asterisks indicate that the values are significantly different from the untreated control (*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; by Student's t test or Mann-Whitney rank sum test). Error bars, S.E.