TABLE 1.
K2P3.1 and K2P9.1 wild type and mutant C terminus sequences used in this study
Amino acid substitutions are shown in boldface type.
| Channel | Experimental system | Sequence of C terminus | Mutation |
|---|---|---|---|
| K2P3.1 human | Electrophysiology (full-length); in vitro kinase assays (C terminus fused to GST) | SLSTFRGLMKRRSSV | Wild type |
| SLSTFRGLMKRRSAV | S393A | ||
| SLSTFRGLMKRRASV | S392A | ||
| SLSTFRGLMKRRAAV | S392A/S393A | ||
| SLSTFRGLMKRRSDV | S393D | ||
| SLSTFRGLMKRRSS. | ΔV394 | ||
| GFP-K2P3.1-HA rat | Immunofluorescence; flow cytometry | SLATFRGLMKRRSSV | Wild type (Ala213-HA-Leu214) |
| K2P9.1 human | Electrophysiology (full-length); in vitro kinase assays (C terminus fused to GST) | SFTDHQRLMKRRKSV | Wild type |
| SFTDHQRLMKRRKAV | S373A | ||
| K2P9.1 rat | In vitro kinase assays (C terminus fused to GST) | TCGENHRLHIRRKSI | Wild type |
| TCGENHRLHIRRKAI | S395A | ||
| GFP-K2P9.1-His rat | Flow cytometry | SLATFRGLMKRRSSV | Wild type (Ala213-His6-Leu214) |