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. 2011 Feb 25;286(16):14137–14145. doi: 10.1074/jbc.M110.176289

FIGURE 1.

FIGURE 1.

Co-expression of p300 dramatically enhances Pax5-mediated induction of the Luc-CD19 reporter expression. Luciferase reporter assays were performed in HEK293 cells by transient transfection with different combinations of vectors as follows: A, Luc-CD19 reporter (0.5 μg), Pax5 (0.5 μg), and p300 (0.5 μg). B, Luc-CD19 reporter (0.5 μg) and Pax5 (0.5 μg) with increasing amounts (0.1, 0.25, or 0.5 μg) p300 expression vectors. C, Luc-CD19 reporter (0.5 μg) and Pax5 (0.5 μg) with the wild-type p300 (0.5 μg) or the p300ΔHAT (0.5 μg) constructs. D, Luc-CD19 reporter (0.3 μg), Pax5 (0.3 μg), and p300 (0.3 μg). After transfection, cells were treated with different concentrations of trichostatin A for 24 h. E, Luc-CD19 reporter (0.3 μg), Pax5 (0.3 μg), and p300 (0.3 μg). After transfection, cells were treated with different concentrations of anacardic acid for 24 h. All inhibitors were added 6 h after transfection. Results presented are the mean values of relative fold induction above the basal level. Error bars indicate mean ± S.D. for triplicate experiments.