FIGURE 5.

Lysine 67 and 87/89 residues within Pax5 are required for p300-mediated enhancement of transcription activation. A, diagram of the functional domains of Pax5 and the relative positions of the acetylated lysine residues. The relative positions of all the lysine residues within Pax5 protein are indicated by numbers, and the identified acetylated lysine residues are shown in red. PRD, paired box DNA binding domain; OP, Oct-peptide; HD, homeolike domain; TA, transactivation domain; ID, inhibition domain. B, different lysine-to-alanine Pax5 mutants were analyzed in luciferase assays in HEK293 cells using the Luc-CD19 reporter construct with or without co-transfection of the p300 expression vector. Results are presented as the mean values of relative fold inductions ± S.D. from triplicate experiments. The expression levels of the Pax5 mutant constructs in each transfection were determined by Western blotting and are shown at the bottom.