FIGURE 2.

Effect of selected compounds that inhibit editosome activity. A, results of the first round of screening using a FRET-based RNA editing assay are shown here. The reactions were performed in the absence (Ø) or presence of various compounds in a 30-μl reaction volume under multiple turnover conditions. Compounds resulting in more than 50% inhibition are indicated by dark gray bars. B, effect of Triton X-100 on the compounds prone to aggregation is shown. Triton X-100 (0.1%, w/v) was used to monitor any nonspecific inhibition due to aggregation. The compounds C35 and S5, which still showed more than 50% inhibition, are indicated by dark gray bars. The error bars represent the experimental variation (S.D.) from three independent repetitions. C, the effect of the best inhibitor compound, C35, on ribozyme activity was evaluated. Cleavage activity of active ribozyme was measured in the absence of the compound (−C35) and presence of the compound (+C35). In all graphs, the y axis represents relative percentages of the cleavage activity for edited ribozyme in each experiment.