FIGURE 1.

pB171 ParM forms filaments in AMP-PNP (A), ATP (B), and GTP (C). pB171 ParM (9.5 μm) was polymerized with 5 mm nucleotide, stained with 0.75% uranyl formate, and visualized by transmission EM. The conditions used were as follows: 100 mm KCl, 30 mm Tris-HCl, pH 7.5, 1 mm MgCl₂, 1 mm DTT, 25 °C. Scale bar, 50 nm.