TABLE 1.

Summary of sedimentation coefficients derived from sedimentation velocity analysis of individual Rab3A and Myo5a tail and the Rab3A/Myo5a tail complex

Protein	S*a	S20,Wb	f/f0c	Calculated mass	Theoretical mass
				kDa	kDa
Rab3A + 1 mm GTPγS	1.9 ± 0.3	2.1	1.4	23.5	24.9
Myo5a tail + 1 mm GTPγS	3.4 ± 1.5	3.6	1.3,	52.9	54.7
	5.1 ± 0.8	5.4	1.3	96.8	109.4
Rab3A/Myo5a tail + 1 mm GTPγS	4.3 ± 1.3	4.6	1.3	73.8	79.6
	5.9 ± 0.6	6.3	1.5	135	134.3

^a S*, experimental sedimentation coefficient determined at 10 °C (± S.E. from two or three independent experiments).

^b S_20,W, standard sedimentation coefficient after correcting for water at 20°C.

^c f/f₀, ratio of the experimental frictional coefficient to the theoretical frictional coefficient for a perfect sphere of the same molecular mass.