TABLE 3.
Comparison of GES-2 with other representative class A β-lactamases
The carbapenemases NMC-A was from Enterobacter cloacae (58), SME-1 from Serratia marcescens (59), and KPC-2 from E. cloacae (60), and the penicillinases TEM-1 from E. coli (54) and SHV-1 from K. pneumoniae (61).
| Enzyme | PDB | Resolution | Rfree | Sequence identitya | R.m.s. deviation values (Å) |
|
|---|---|---|---|---|---|---|
| All Cαb | Core Cα | |||||
| Å | ||||||
| GES-2c | This work | 1.65 | 0.193 | |||
| GES-1 | 2qpn | 1.10 | 0.182 | –d | 0.36 (266) | 0.28 |
| NMC-A | 1bue | 1.64 | 0.214 | 34% | 1.3 (246) | 0.81 |
| SME-1 | 1dy6 | 2.13 | 0.244 | 36% | 1.3 (251) | 0.81 |
| KPC-2 | 3dw0 | 1.60 | 0.211 | 36% | 1.2 (240) | 1.02 |
| TEM-1 | 1zg4 | 1.55 | 0.240 | 34% | 1.1 (238) | 0.71 |
| SHV-1 | 1vm1 | 2.02 | 0.257 | 32% | 1.4 (250) | 0.87 |
a Not including the leader sequence.
b Values in parentheses give the total number of Cα atoms matched. The core (155 Cα atoms) comprises residues 42–48, 61–90, 119–193, 199–217, 230–238, 243–250, and 259–265.
c The coordinates used are for the GES-2·tazobactam complex.
d The difference between GES-1 and GES-2 is the amino acid residue at position 170 (glycine in GES-1 and asparagine in GES-2).