TABLE 2.

Activity of CurM TE variants

	Activitya
	%
WT	100
R205Q	1.8 ± 0.3
R205E	2 ± 2
R205A	6 ± 3
N267A	34 ± 3
N211A	55 ± 2
H266R	1.4 ± 0.2

^a Raw HPLC chromatogram peak area for the holo-ACP product with background subtracted and normalized with an internal standard to correct for differences in sample injection. The area for each mutant was normalized to the wild type within each replicate. Mean ± S.D. from duplicate experiments are shown.