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Proposed pathway for the steps in nucleotide selection by KF preceding phosphodiester bond formation. EO indicates the KF-DNA complex in which the fingers-open conformation dominates, and EC is the complex in which the fingers-closed conformation dominates. DNA* refers to the DNA rearrangement reported by 2-aminopurine (2-AP) at position N = +1 of the template strand (4). The scheme was adapted from Refs. 1–4.
