FIGURE 5.
Transferase-specific threonine enhancement products roughly correlate with experimental transferase activity. Panels A–C, plots of glycosyltransferase activity (gray) and transferase-specific Thr enhancement product sums (black) for the peptides Muc1a, Muc1b, Muc2, Muc7, OSM, EA2, gp120, and fibronectin whose activity against ppGalNAc T1 (panel A), ppGalNAc T2 (panel B), and ppGalNAc T3 (panel C) was taken from Bennett et al. (65). Peptide sequences for panels A–C are defined in supplemental Table 1. Panels D–F, plots of glycosyltransferase activity (gray) and transferase-specific Thr enhancement product sums (black) for the peptides mOPN1 to mOPN3, mBSP1 to mBSP7, EA2, and gp120 whose activity against ppGalNAc T1 (panel D), ppGalNAc T2 (panel E), and ppGalNAc T3 (panel F) was taken from Miwa et al. (63). Peptide sequences for panels D–F are defined in supplemental Table 2. Enhancement value products were obtained as described under “Experimental Procedures.”