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. 2011 Feb 14;286(16):14508–14515. doi: 10.1074/jbc.M110.190165

FIGURE 4.

FIGURE 4.

GGPPS mediates the regulation of Erk1/2 activity by Egr-1 in insulin-resistant adipocytes. A, insulin-resistant adipocytes were treated with insulin (Ins, 1000 nm) for the indicated times with changing fresh medium containing insulin every 12 h. The cell lysates were Western blotted against GGPPS. B, insulin-resistant adipocytes were infected with GFP-dnEgr-1 adenovirus or transfected with Egr-1 siRNA (siEgr-1) for 48 h. After insulin treatment for 3 h, the cells were lysed, and total RNA was collected and subjected to quantitative PCR for GGPPS mRNA. C, adipocytes were infected with GFP-Egr-1 adenovirus and GGPPS siRNA (siGGPPS) adenovirus or treated with GGTI. After 48 h, the cells were Western blotted against Erk. D and E, IR adipocytes were infected with GGPPS siRNA adenovirus (E) or a scrambled control adenovirus (D) for 48 h and then subjected to long term insulin stimulation. The cell lysates were collected at the indicated times and subjected to Western blot analysis. **, p < 0.01, compared with the group untreated with insulin; ##, p < 0.01, compared with the group treated with insulin. P-Erk1/2, phosphorylated Erk1/2; T-Erk1/2, total Erk1/2.