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. 2011 Feb 14;286(16):14508–14515. doi: 10.1074/jbc.M110.190165

FIGURE 5.

FIGURE 5.

Ras prenylation is crucial for Egr-1/GGPPS-promoted Erk1/2 activity. A and B, adipocytes were infected with Egr-1 or GGPPS adenovirus or disrupted prenylation by knockdown GGPPS or treated with GGTI for 48 h. The cells were subjected to Ras immunoprecipitation and prenylation measurement (A) or Western blotted to detect changes in Erk1/2 activity (B). C and D, IR adipocytes were infected with dnEgr-1 or GGPPS siRNA adenoviruses for 48 h. After insulin (Ins) treatment for 3 h, the cells were subjected to Ras immunoprecipitation and prenylation measurement (C) or Western blotted to detect changes in Erk1/2 activity (D). IP, immunoprecipitation; IB, immunoblot; P-Erk1/2, phosphorylated Erk1/2; T-Erk1/2, total Erk1/2.