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. 2011 Feb 28;286(16):14628–14638. doi: 10.1074/jbc.M110.195461

FIGURE 1.

FIGURE 1.

RET is cleaved by caspase in SCG neurons during trophic starvation, and a caspase-truncated fragment of RET is stabilized. A, schematic representation of the RET structure with its different domains. Arrows indicate the Asp-707 and Asp-1017 (human sequence) caspase cleavage site. B, Western blot (WB) analysis of extracts from SCG neurons treated for 24 h with NGF, GDNF, GDNF plus soluble GFRα1, anti-NGF (−), or anti-NGF with the caspase inhibitor BAF and immunostained with intracellular RET-derived antibody. Arrow indicates full-length RET. C, left panel, time course of RET cleavage in extracts of SCG neurons analyzed by Western blotting using extracellular RET-derived antibody. Solid arrow indicates full-length RET, and open-headed arrow indicates the presence of caspase-truncated RET. C, right panel, quantification of levels of caspase-truncated RET compared with actin. D, upper panel, Western blot of SCG neurons extracts treated for 12 h with NGF, GDNF and soluble GFRα1, anti-NGF, or anti-NGF plus caspase inhibitor BAF and stained with extracellular RET-derived antibody. Solid arrow indicates full-length RET, and open-headed arrow indicates the presence of caspase-truncated RET. D, lower panel, same extracts were used for active caspase-3 Western blotting. E, upper panel, Western blot of SCG neuron extracts treated for 24 h with NGF, GDNF and soluble GFRα1, anti-NGF, or anti-NGF plus caspase inhibitor BAF and stained with extracellular RET-derived antibody. Solid arrow indicates full-length RET, and open-headed arrow indicates the presence of caspase-truncated RET. E, lower panel, same extracts were used for an active caspase-3 immunoblotting.