Sequence alignment of S. cerevisiae Gwt1p, mouse PIG-W, and their orthologs. Four regions that were highly homologous among 11 species are depicted. Identical amino acid residues among all 11 species are highlighted. Conservative residues among all 11 species are shaded. Positions that were mutated to alanine in S. cerevisiae Gwt1p are marked with asterisks above the sequences; positions mutated in mouse PIG-W are marked below the sequence. Sc, S. cerevisiae; Ca, C. albicans; Af, A. fumigatus; Nc, N. crassa; Cn, C. neoformans; Pf, P. falciparum; Ce, C. elegans; Ci, Ciona intestinalis; Xl, X. laevis; Hs, H. sapiens; Mm, M. musculus.