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. 2011 Mar 8;286(16):14681–14690. doi: 10.1074/jbc.M110.201061

FIGURE 4.

FIGURE 4.

Nampt gene is down-regulated in liver of LTKO mice. A and B, Sirt1 activity was assessed in the liver of control and LTKO mice by an acetylation analysis of PGC-1α and a fluorometric assay, respectively (n = 3). AFU, artificial fluorescence unit. C–E, NAD+ and NADH levels were measured in the liver of control and LTKO mice (n = 5). NAD+/NADH ratios were calculated according to the NAD+ and NADH values. F, expression of genes involved in NAD+ biosynthesis was analyzed in the liver of control and LTKO mice (n = 3) treated with a high fat diet for 3.5 months by real time RT-PCR. G, expression of heme decycling genes was analyzed in the liver of control and LTKO mice (n = 3) treated with a high fat diet for 3.5 months by real time RT-PCR. H, Nampt mRNA levels were analyzed in the liver of control and LTKO mice (n = 3) at ZT (under regular light/dark cycles, the time of lights on is ZT0 and the time of lights off is ZT12) 5, 11, and 16. I and J, Nampt protein was analyzed in the liver of control (Con) and LTKO (KO) mice at ZT 1, 6, 10, and 16. Both quantitative analyses (I) and immunoblots (J) are presented. * indicates significance with p < 0.05 in control versus LTKO. Error bars indicate S.E.