Skip to main content
. 2011 Mar 8;286(16):14681–14690. doi: 10.1074/jbc.M110.201061

FIGURE 5.

FIGURE 5.

Nampt gene is a target of FoxOs. A and B, Nampt mRNA and protein analyses in mouse primary hepatocytes transduced with adenoviruses carrying GFP and FoxO1 overexpression or knockdown (shRNA) constructs (n = 3). C, a schematic diagram of the proximal promoter of the mouse Nampt gene. Previously identified E-box elements and a putative IRE (IRE1) are indicated. TSS, transcription start site. D, a sequence alignment among mouse, rat, and human NAMPT gene promoters at the putative IRE1 site. E and F, luciferase (Luc) reporter assays using serial deletion constructs of the mouse Nampt gene promoter and FoxO1/3-, Clock-, and Bmal1-overexpressing plasmids (n = 3). G and H, chromatin immunoprecipitation analysis of the putative IRE1 and an upstream IRE2 by regular and real time PCRs, respectively (n = 3). I, Nampt mRNA analysis in mouse primary hepatocytes transduced with GFP- or FoxO1-overexpressing adenoviruses in the absence or presence of insulin (n = 3). * indicates significance with p < 0.05. Error bars indicate S.E.