Nampt regulates lipid metabolism in mouse primary hepatocytes. A–D, protein, NAD+, and intracellular TG levels were analyzed in mouse primary hepatocytes that overexpressed GFP, Sirt1, Foxo1, and Nampt for 48 h using corresponding adenoviruses. E and F, de novo lipogenesis and fatty acid (FA) oxidation analyses in control and LTKO mouse primary hepatocytes that overexpressed GFP, Sirt1, Foxo1, and Nampt, respectively. * indicates significance with p < 0.05 between GFP and other gene overexpression in control or LTKO hepatocytes (n = 3). # indicates significance with p < 0.05 between control and LTKO hepatocytes (n = 3). Error bars indicate S.E.