FIGURE 3.

Tumor-promoting role of the MT1-MMP-induced Warburg effect in MDA-MB-231 cells, as determined by in vitro experiments. Mint3 expression was knocked down in MDA-MB-231 cells using Mint3 shRNA (shMint3). Control cells were transfected with LacZ shRNA (shLacZ). The effect on the following parameters was assessed. A, Mint3 protein expression (left panel) and HIF-1α protein expression in response to hypoxia (right panel); B, HIF-1α protein expression during normoxia; C, VEGF and GLUT1 mRNA expression; D, 2-NBDG intake (left panel), lactate production (middle panel), and ATP production in the presence or absence of the glycolysis inhibitor, 2-deoxyglucose (right panel); E, in vitro cell proliferation during normoxia (left panel) and hypoxia (right panel). The error bars indicate S.D. (n = 3), and the data were analyzed by the t test. IP, immunoprecipitation; IB, immunoblot. **, p < 0.01.