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. 2011 Mar 8;286(16):14724–14736. doi: 10.1074/jbc.M110.214973

FIGURE 8.

FIGURE 8.

Cellular localization and aggregation of disease-associated mutants. A, shown is surface immunofluorescence staining of PrP on HEK cells stably expressing WT, PG14, P101L, P104L, P104S, P104T, G113V, A116V, M128V, G130V, or D177N PrP or empty vector. Scale bar = 50 μm. B, shown is quantitation of cell surface PrP for each of the mutants (mean ± S.E., n = 3 independent experiments) by cell blotting. Examples of total and surface signals are shown below each bar. C, quantitation of the percentage of insoluble PrP (mean ± S.E., n = 3 independent experiments) is shown. Western blots of PrP in detergent-soluble (S) and insoluble (I) fractions are shown below each bar. Asterisks (*) indicate values that are significantly greater than those for WT PrP (p < 0.05, one-tailed Student's t test). Plots of cell death versus cell surface PrP (D) or cell death versus detergent insolubility (E) for each disease-associated point mutant show no significant correlations.