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. 2011 Apr 20;100(8):1969–1976. doi: 10.1016/j.bpj.2011.02.061

Figure 1.

Figure 1

Changes in fluorescence intensity during the chase phase of the single nucleotide turnover experiments are shown for mantATP chased by ATP (O), and for ATP chased by mantATP (●). In both experiments the intensity changes in two phases—a rapid phase with a time constant of ∼10–15 s and a slow phase with a time constant of ∼140 s. The symmetry between the two experiments shows that the single nucleotide turnover measurements are the same for mantATP replacing ATP and for ATP replacing mantATP. The data were fit with a double-exponential function (solid lines). Parameters of the fits to the data shown here are provided in the Supporting Material.