Figure 4.

Bundle of cardiac cells visualized by spinning-disk confocal microscopy after 1.5 min in the chase phase. The cells were incubated in a solution containing 125 μM mantATP for 2 min then chased with a solution containing 4 mM ATP for 1.5 min before taking the image. The horizontal stripes are ∼1.5 μm in width, close to the length of the A-band in cardiac muscles, showing that much of the fluorescence intensity remaining in the muscle is associated with the A-band. The sarcomere length is 2.1 μm. The bar represents 10 μm.