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. 2011 Apr 20;100(8):2053–2062. doi: 10.1016/j.bpj.2011.02.015

Figure 10.

Figure 10

Localizations of type I and II collagens in engineered cartilage tissues through immunohistochemical staining, peptide, and methylene pitch-angle imaging. (A, Red) SHG from collagen and (A, green) autofluorescence from chitosan scaffold. (B and C) Immunohistochemical images for type I and II collagen, respectively. (Yellow arrows) Type I collagen (B) and type II collagen (C). (D and E) Peptide and methylene pitch-angle imaging. (F and G, histograms) Peptide and methylene pitch-angles in the dashed-circled region, respectively. (H) Summary of the peptide and methylene pitch-angles for type I and II collagen in engineered cartilage tissue.