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. 2011 Apr 20;100(8):2053–2062. doi: 10.1016/j.bpj.2011.02.015

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© 2011 by the Biophysical Society.

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Three-dimensional SHG imaging and second-order susceptibility analysis of trachea cartilage. (A and B) Three-dimensional SHG image stack (95 × 95 × 30 μm³) of trachea cartilage. (C) SHG image of a section at 15 μm below the surface (indicated by dashed lines in A and B). Boxed region indicates region of well-aligned fiber used for localized second-order susceptibility analysis. (D) The definition of fibril orientation. (E–I) Histograms of fibril orientation angle, χ_xxz/χ_zxx, χ_zzz/χ_zxx, peptide pitch-angle θ^(p), and methylene pitch-angle θ^(m), respectively. Mean and standard deviations are summarized at Table 1 .