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. 2011 Apr 20;100(8):2006–2015. doi: 10.1016/j.bpj.2011.02.049

Figure 2.

Figure 2

Force-extension curves of pBACgus11 (A and C) and piJ702 (B and D) in the presence of spermine (A and B) and ethanol and spermine (C and D). An individual dsDNA molecule is first stretched and relaxed in TE buffer (black line; [NaCl] and [spermine], as displayed in the legends), with subsequent cycles (red and blue lines) performed in a mixture of the same buffer with ethanol (% concentration as displayed in the legends). Stretch and release curves are shown in the same color. (A and B, insets) Zooms of the low-force region to show the strength of the condensation plateaus, with gray arrows marking the stretch and relax paths. Pulling speed, 500 nm/s. spm, spermine.