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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Nat Chem Biol. 2010 May 2;6(6):433–441. doi: 10.1038/nchembio.367

Figure 2. EFOX-D5 is an α,β-unsaturated oxo-derivative of DPA.

Figure 2

a, Characteristic BME-EFOX fragmentation pattern derived from the EPI of BME-EFOX-D5. b, EFOX-D5 levels in activated RAW264.7 cells grown for 3 days in medium supplemented with the indicated FA. ND indicates not detectable. c, Diagram of NaBH4 reaction. d, MRM scans monitoring for the m/z transitions 343.2/299.2 (13-EFOX-D5/loss of CO2; upper and middle panel) and 345.2/327.2 (hydroxy-DPA/loss of H2O; lower panel) in RAW264.7 cell lysates purified for EFOX-D5 ± NaBH4. e, MS/MS fragmentation of EFOX-D5 purified from activated RAW 264.7 cells and reduced with NaBH4.