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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Nat Chem Biol. 2010 May 2;6(6):433–441. doi: 10.1038/nchembio.367

Figure 4. EFOXs form adducts with proteins and GSH following activation of RAW264.7.

Figure 4

a, Cell lysates from activated RAW264.7 cells were split into two groups: treatment with BME followed by protein precipitation with acetonitrile (“Total”) and protein precipitation followed by BME treatment (“Free + small molecule adducted”). b, Chemical structure and fragmentation pattern of GS-13-EFOX-D5. c, Chromatographic profiles and positive ion mass spectra of GSH adducts of 13-EFOX-D5 and 17-EFOX-D5 derived from standards (upper panels), cell medium (middle panel) and cell pellet (lower panel), N/A profiles correspond to non-activated cell samples. Grey chromatograms represent GS-17-EFOX-D5 (upper panel left) and GS-13-EFOX-D5 (upper panel right) standards. Fragments 345.3 and 523.3 were monitored in cell media and cell pellet samples respectively.