Figure 3.

Impact of copy number alterations on the mRNA expression of CCNE1 in OS cell lines. Relative expression values for samples with copy number loss, no copy number change, gain, and amplification are shown in a heatmap generated using Genesis software.¹⁸ Copy number status was determined as follows: loss, fewer than two copies; neutral, two copies; gain, more than two copies; and amplification, 5 or more copies. Expression data were normalized and scaled from 0 to 100.