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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: J Immunol. 2009 May 15;182(10):5960–5969. doi: 10.4049/jimmunol.0900008

FIGURE 5.

FIGURE 5

Lentivector immunization dramatically increases T cell infiltration into established B16F0 tumors. C57BL/6 mice were inoculated with 1 × 105 B16F0 cells. Five days later, mice were immunized with 2.5 × 107 TU of muTRP1-lv or left alone (five mice in the treatment group and three mice in control group). Fifteen days after immunization (20 days after tumor inoculation), tumors were collected. A, Half portion of each tumor was examined for TIL by flow cytometry. Flow cytometry gates were set on the live cells based on the size (forward scatter). Thy1.2 was used to stain the T cells, which were then further analyzed for CD8 and CD4 markers. B, Summary of data from all the mice is shown, including the normalized total T cells per gram of tumor and ratio changes of CD8 vs CD4 T cells. C, The other half of tumor was used for immunofluorescent staining of CD8 and CD4 T cells.