Figure 6.

Arabidopsis DNA Pol λ, PCNA1, and PCNA2 Colocalize in the Nucleus of Plant Cells.

Images of tobacco BY-2 protoplasts 24 h after cotransfection with constructs encoding GFP-AtPCNA1 and RFP-AtPCNA2 ([A] to [D]) or GFP-AtDNA pol λ and RFP-AtPCNA2 ([E] to [G]) constructs. Both DNA pol λ ([B] and [F]) and PCNA1 (A) and PCNA2 (E) are targeted to the nucleus, as previously reported (Raynaud et al., 2006). Digital merging of images reveals the colocalization of DNA pol λ both with PCNA1 (C) and with PCNA2 (G). Respective bright-field differential interference contrast (DIC) images are shown ([D] and [H]). Bars = 25 μm.