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. 2011 Feb 25;23(2):567–582. doi: 10.1105/tpc.110.080754

Figure 5.

Figure 5.

Loss of the REV MEKHLA Domain Results in More Severe Overexpression Phenotypes.

(A) Phenotypic analysis of stably transformed Arabidopsis plants. T1 seedlings were scored for curled up cotyledons caused by ectopic REV activity. miRNA-resistant REV is designated REV*. Asterisks indicate transgenic lines that are statistically different from 35S-REV* plants (Z test, confidence > 95%).

(B) Phenotypic analysis of stably transformed Arabidopsis plants. First to fifth rosette leaves of T1 seedlings were scored for abaxial (lower) leaf flaps. miRNA-resistant REV is designated REV*. Asterisks indicate transgenic lines that are statistically different from 35S-REV* plants (Z test, confidence > 95%).

(C) Row 1: Schematic representation of the REV protein forms overexpressed in the tissue samples below. Row 2: Abaxial side of the first rosette leaf of representative transgenic Arabidopsis plants. The arrows point to leaf flaps. Bar = 2 mm. Row 3: Sepals of representative transgenic Arabidopsis plants. Bar = 2 mm. Row 4: Pistils of representative transgenic Arabidopsis plants. The arrows point to abaxial ovules. Bar = 2 mm.