Figure 1.

RNA Gel Blot, 2D, and Immunoblot Analyses of the Expression of the ABR1 Protein and Gene in Pepper Leaves Infected by Xcv or Treated with ABA and SA.

(A) Identification of the ABR1 protein by 1D and 2D electrophoresis and immunoblot analysis. The red circles and rectangles indicate ABR1 expression. IB, imunnoblotting.

(B) Organ-specific expression of ABR1 in pepper plants. rRNA is used as loading control ([B] to [E]).

(C) Expression of ABR1 in leaves at various times after treatment with 100 μM ABA. H, healthy leaves.

(D) Expression of ABR1 and PR1 in leaves at various times after treatment with 5 mM SA. Pepper basic pathogenesis-related protein gene (PR1) was used as a comparable control. H, healthy leaves.

(E) Expression of the ABR1 gene in pepper leaves at various times after inoculation with the virulent (compatible) strain Ds-1 and the avirulent (incompatible) strain Bv5-4a of Xcv. H, healthy leaves.

(F) Immunoblot analysis of expression of ABR1 protein in leaves at various times after inoculation with the Ds-1 and Bv5-4a strains of Xcv. Immunoblotting used a specific antiserum raised against an ABR1 peptide. H, healthy leaves; IB, imunnoblotting; CBB, Coomassie blue.

[See online article for color version of this figure.]