Figure 2.

Effects of Agrobacterium-Mediated Transient Epression of ABR1 on the Cell Death Response of Pepper Leaves.

(A) Immunoblot analysis of transiently expressed myc-ABR1 in leaves at different time points after agroinfiltration. An anti-myc antibody was used to detect myc-ABR1 on the immunoblot. IB, imunnoblotting.

(B) Induction of the cell death response by transient expression of ABR1. The leaf areas between the lateral veins received the indicated Agrobacterium strain at the indicated OD₆₀₀. Photographs were taken 36 h after agroinfiltration. The experiment was performed three times with similar results. Visible, visible light image; trypan blue, trypan blue staining.

(C) Electrolyte leakage from leaf discsagroinfiltrated with the empty vector control and the ABR1 transient expression construct (OD₆₀₀ = 0.2).

(D) Accumulation of H₂O₂ in leaves transiently expressing ABR1.

(E) and (F) Quantification of ABA (E) and SA (F) levels in empty vector control leaves and in leaves transiently expressing ABR1 after agroinfiltration (OD₆₀₀ =0 .5). FW, fresh weight. Data are the means ± sd (n = 3) from three independent experiments. Asterisks indicate a significant difference, as determined by the two-tailed t test (P < 0.05) ([C] to [F]).

[See online article for color version of this figure.]