Figure 6.

Enhanced Susceptibility of ABR1-Silenced Pepper Leaves to Xcv Infection.

(A) Relative expression of ABR1 transcript using real-time RT-PCR analysis. dai, days after inoculation.

(B) and (C) Disease symptoms induced on leaves of empty vector control (TRV:00) or ABR1-silenced (TRV:ABR1) pepper plants 0, 3, and 5 d after inoculation (dai) with the Xcv virulent (compatible) strain Ds1 ([B], left panel) and avirulent (incompatible) strain Bv5-4a ([C], left panel) (10⁶ colony-forming units [cfu] mL⁻¹). Bacterial growth in leaves inoculated with strain Ds1 ([B], right panel) and strain Bv5-4a ([C], right panel) (10⁴ cfu mL⁻¹). Trypan blue staining of leaves ([C], bottom left panel) and electrolyte leakage from leaf discs ([C], bottom right panel) of empty vector and ABR1-silenced plants inoculated with strain Bv5-4a (10⁷ cfu mL⁻¹). Error bars indicate sd (n = 3) from three independent experiments. Asterisks indicate a significant increase in bacterial growth and electrolyte leakage, as determined by the two-tailed t test (P < 0.05) ([A] to [C]). Bar = 500 μm.

[See online article for color version of this figure.]