Figure 9.

Enhanced Resistance of ABR1-OX Transgenic Arabidopsis Plants to Pst Infection.

(A) Constitutive protein expression of ABR1 in three lines (#1, #2, and #3) of transgenic plants by immunoblotting (IB). WT, wild type.

(B) Spontaneous cell death response in the transgenic leaf tissue. Bars = 100 μm.

(C) DAB (1 dai, left), aniline blue (1 dai, middle), and trypan blue staining (2 dai, right) of leaves of wild-type and ABR1-OX plants inoculated with Pst DC3000 and Pst DC3000 (avrRpm1) (10⁷ cfu mL⁻¹). The number of calloses per mm² is represented with the means ± sd (n = 3) in the box. Bars = 100 μm.

(D) Bacterial growth in leaves of wild-type and ABR1-OX plants inoculated with Pst DC3000 and Pst DC3000 (avrRpm1) (5 × 10⁴ cfu mL⁻¹). Data are the means ± sd (n = 3) from three independent experiments. Different letters and asterisks indicate a significant difference, as determined by Fisher’s protected LSD test (P < 0.05) and the two-tailed t test (P < 0.05), respectively ([D] to [F]).
(E) Accumulation of H₂O₂ in ABR1-OX transgenic Arabidopsis.

(F) Electrolyte leakage from seven leaf discs (7 mm in diameter) of wild-type and ABR1-OX transgenic Arabidopsis plants.

[See online article for color version of this figure.]