Table 1.
Proteins Differentially Expressed in Pepper Leaves Infected by Virulent (Compatible) Strain Ds1 and Avirulent (Incompatible) Strain Bv5-4a of Xcv, as Identified by MALDI-TOF MS and LC/MS-MS
| Spot No. | Accession No. | Experimental pl/MM (kD) | Theoretical pI/MM (kD) | SC (%) | MS Used for Identification | Spot Density |
|||
| Protein (Organism) | Healthy | Compatible | Incompatible | ||||||
| Novel protein | |||||||||
| I. RNA synthesis | |||||||||
| N1 | RNA binding protein precursor (Persea americana) | CAD18921 | 4.29/33 | 4.49/32.9 | 10 | LC-MS | 0a | 0.1045b | 0.0644b |
| II. Disease, virulence, and defense | |||||||||
| N2 | Pathogenesis-related protein 10 (C. annuum) | AAF63519 | 5.31/24 | 5.2/17.38 | 16 | LC-MS | 0a | 0.0932a | 1.2633b |
| N3 | ABA-responsive protein (C. annuum) | CA524559 | 6.29/38 | 8.96/13.01 | 30 | LC-MS | 0a | 0.0171a | 0.5689b |
| N4 | Precursor β-1,3-glucanase (Nicotiana plumbaginifolia) | CAA38540 | 7.96 / 32 | 7.79/39.96 | 10 | LC-MS | 0a | 0.8030b | 0.8050b |
| N5 | Precursor β-1,3-glucanase (N. plumbaginifolia) | CAA38540 | 8.19/32 | 7.79/39.96 | 10 | LC-MS | 0a | 0.3080c | 0.0543b |
| N6 | Glucan endoglucosidase (Nicotiana tabacum) | 1410344A | 7.34/32 | 6.6/39.13 | 13 | LC-MS | 0a | 0.0776b | 0.0568b |
| N7 | Peroxidase (C. annuum) | AAL35364 | 7.47/34 | 6.88/36.05 | 26 | LC-MS | 0a | 0.2630b | 0.2770b |
| N8 | Peroxidase (C. annuum) | AAL35364 | 7.46/33 | 6.88/36.05 | 29 | LC-MS | 0a | 0.0123a | 0.1020b |
| N9 | Endochitinase (C. annuum) | AAY90154 | 6.99/31 | 7.7/19.13 | 58 | LC-MS | 0a | 0.0199b | 0.0497c |
| N10 | Osmotin-like protein precursor (Pathogenesis-related protein PR-5d) (N. tabacum) | P25871 | 7.00/26 | 6.49/27.63 | 5 | LC-MS | 0a | 0.1150b | 0.0900b |
| N11 | TMV induced protein 1-2 (C. annuum) | AAO49266 | 7.00/20 | 5.5 / 17.87 | 51 | LC-MS | 0a | 0.0424b | 0.1130c |
| Upregulated protein | |||||||||
| I. C-compound and carbohydrate metabolism | |||||||||
| U1 | Acetyl-CoA carboxylase (Triticum aestivum) | AAC49275 | 6.48/21 | 6/252.86 | 6 | MALDI-TOF | 0.1580a | 0.4973b | 0.4288b |
| II. Photosynthesis | |||||||||
| U2 | Light-harvesting chlorophyll a/b binding protein (Nicotiana sylvestris) | BAA25388 | 4.95/29 | 5.2/28.29 | 13 | MALDI-TOF | 0.0494a | 0.1038b | 0.1642c |
| III. Extracellular/secretion protein | |||||||||
| U3 | Thioredoxin (C. annuum) | AAR83852 | 4.91/16 | 5.01/13.71 | 20 | LC-MS | 0.0821a | 0.1646c | 0.0119b |
| IV. Disease, virulence, and defense | |||||||||
| U4 | Putative pathogenesis-related protein (Capsicum chinensis) | CAI48023 | 5.22/21 | 5.22/17.27 | 18 | LC-MS | 0.0649a | 0.7988b | 0.7090b |
| V. Unclassified protein | |||||||||
| U5 | Chloroplast protease (C. annuum) | CAA09935 | 5.05/81 | 5.8/74.15 | 26 | MALDI-TOF | 0.0593a | 0.1270b | 0.1462b |
| U6 | Chloroplast protease (C. annuum) | CAA09935 | 5.29/81 | 5.8/74.15 | 39 | MALDI-TOF | 0.0732a | 0.2013b | 0.1617b |
| U7 | JH02012D09 JH-201 C. annuum cDNA 5′, mRNA sequence (C. annuum) | CO776410 | 5.03/37 | 9.65/16.29 | 20 | LC-MS | 0.2840a | 0.4007b | 0.5081b |
| Downregulated protein | |||||||||
| I. C-compound and carbohydrate metabolism | |||||||||
| D1 | Glyceraldehyde-3-phosphate dehydrogenase (C. annuum) | CAC80374 | 6.26/47 | 6.3/33.13 | 21 | MALDI-TOF | 0.1470a | 0.0493b | 0.0866c |
Spot density is expressed as the percentage volume to correct the variability due to silver staining, which is calculated by ImageMaster 2D Platinum 6.0 (Amersham Biosciences). Significant differences were determined by Student's t test (P ≤ 0.05, labeled a to c). SC, sequence coverage; MM, molecular mass.