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. 2011 Feb 9;23(2):661–680. doi: 10.1105/tpc.110.081802

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© 2011 American Society of Plant Biologists

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Figure 5. — FH5 Nucleates Actin Assembly from G-Actin and the Profilin/Actin Complex.

(A) and (B) Time course of actin polymerization in the presence of FH5 FH2 (A) or FH5 FH1FH2 (B) monitored by pyrene fluorescence. Various concentrations of FH5 FH2 or FH5 FH1FH2 were added to 2 μM 5% pyrene-labeled actin before the initiation of actin polymerization.

(C) Nucleation efficiency of FH5 FH2 and FH5 FH1FH2. The efficiency was calculated at half-maximal actin polymerization according to Blanchoin et al. (2000).

(D) FH5 FH2 was not able to nucleate actin from the profilin/actin complex. The reactions were conducted with different combinations as indicated at right.

(E) FH5 FH1FH2 was able to nucleate actin assembly from the profilin/actin complex. The reactions were conducted with different combinations as indicated at right.