Figure 3.

Respiratory Parameters in Leaves of Antisense Succinate Dehydrogenase Tomato Plants.

(A) Dark respiration measurements performed in 4- to 5-week-old plants. WT, wild type.

(B) Ratio of carbon dioxide evolution from C3,4 to C1 positions of glucose in leaves of antisense SDH tomato plants.

The leaf discs were taken from 4-week-old plants and were incubated in 10 mM MES-KOH solution, pH 6.5, 0.3 mM glucose supplemented with 2.32 kBq mL⁻¹ of [1-¹⁴C]- or [3,4-¹⁴C]-glucose at an irradiance of 200 μmol m⁻² s⁻¹. The ¹⁴CO₂ liberated was captured (at hourly intervals) in a KOH trap, and the amount of radiolabel released was subsequently quantified by liquid scintillation counting. Values are presented as means ± se of determinations on six individual plants per line. An asterisk indicates values that were determined by the Student’s t test to be significantly different (P < 0.05) from the wild type.