Table 1.
Respiratory State 3 Activities of Mitochondria Isolated from Tomato Fruits from Wild-Type or Succinate Dehydrogenase Antisense Lines
| O2 Consumption (nmol O2 min−1 mg−1 Protein) |
||||
| Substrate | Wild Type | SDH14 | SDH43 | SDH52 |
| NADH | 142.41 ± 21.32 | 131.16 ± 16.57 | 127.61 ± 19.14 | 136.72 ± 24.88 |
| Malate | 108.23 ± 15.39 | 98.71 ± 14.27 | 102.36 ± 11.95 | 107.79 ± 16.52 |
| Citrate | 134.12 ± 12.45 | 127.29 ± 14.19 | 135.24 ± 16.01 | 133.14 ± 15.28 |
| 2-Oxoglutarate | 145.27 ± 8.71 | 138.97 ± 9.85 | 140.35 ± 10.15 | 134.52 ± 14.57 |
| RCR | 2.86 ± 0.28 | 2.45 ± 0.46 | 2.48 ± 0.25 | 2.94 ± 0.72 |
| ADP/O | 0.96 ± 0.19 | 0.85 ± 0.33 | 0.77 ± 0.27 | 0.92 ± 0.25 |
Mitochondrial protein (50 to 100 μg) was used for the respiratory measurements. Data are presented as mean values ± se and are averages from three to five different mitochondrial isolations from tomato fruits 35 d after flowering.