Phenotypes of FH5/RMD RNAi Lines.
(A) The FH5/RMD RNAi plants display varied levels of reduced height. Ri-s, strong phenotype, 35.9 ± 2.5 cm, n = 25; Ri-m, moderate phenotype, 44.9 ± 2.7 cm, n = 34; and Ri-w, weak phenotype, 80.3 ± 5.4 cm, n = 11. WT, wild type. Bar = 20 cm.
(B) and (C) The bending growth of seedling (B) and curled panicle branches (C) shown in Ri-s.
(D) qRT-PCR analysis of FH5/RMD in RNAi plants (error bars represent sd).
(E) RT-PCR analysis of FH12, a gene sharing high sequence similarity to FH5 in the RNAi lines, ACTIN as control.
[See online article for color version of this figure.]