Figure 8.

Time-Lapse Fluorescence Microscopy Visualization of the Effect of FH5 Protein on Actin Filament Elongation from the Actin/Profilin Complex.

Oregon-green-actin/profilin complex (1 μM actin and 2 μM profilin) was polymerized in the absence or presence of 200 nM varoius formin proteins. Frames were taken at the indicated time during polymerization.

(A) to (P) Time-lapse micrographs of Oregon-green-actin/profilin polymerization with no formin ([A] to [D]; see Supplemental Movie 1 online), FH5 FH1FH2 ([E] to [H]; see Supplemental Movie 2 online), FH5 FH2 ([I] to [L]; see Supplemental Movie 3 online), and AFH1 FH1FH2 ([M] to [P]; see Supplemental Movie 4 online). Bars = 5 μm, and arrows indicate the position of actin filament ends over time.

(Q) Elongation rates (±se) of actin filaments in the absence or presence of FH5 FH1FH2, FH5 FH2, or AFH1 FH1FH2.