Figure 1.

Changes of Histone Modifications on phyA in the D/L Transition.

(A) phyA expression levels in the D/L transition. Two-week-old Arabidopsis plants grown under long-day conditions (16 h of L/8 h of D) were harvested at the end of the L period (L1) and followed by D/L treatment for 24-h intervals (D1, L2, and D2). PhyA expression levels were determined by quantitative RT-PCR with a gene-specific primer set.

(B) Schematic diagram of phyA gene structure. Promoter (P), exons (e1, e2, e3, and e4), and 5′ UTR (5′U) and 3′ UTR (3′U) are represented by black, gray and white boxes, respectively. The amplification sites for ChIP analyses are indicated as numbers (1–11) with black bars below the map. ATG is the translation start site.

(C) RNA Pol II binding affinity on the phyA locus (regions 3, 7, and 11) during the D/L transition. The relative enrichment of RNA Pol II binding affinity was determined using anti-Pol II antibody with region-specific primers.

(D) and (E) Relative levels of histone H3 modifications (K4me2, K4me3, K9/14ac, K9me2, K9me3, K27ac, K27me3, and K36me3) at region 3 (D) and region 7 (E) in the D/L transition.

(F) Relative levels of histone H3 acetylation (K9ac and K14ac) in the D/L transition.

(G) Relative levels of histone H4 acetylation (K5ac, K8ac, K12ac, and K16ac) in the D/L transition.

Error bars indicate sd (n = 3).