Figure 4.

Changes of Histone Modifications on phyA during the Diurnal Cycle.

(A) and (B) Relative CCA1 (A) and phyA (B) expression levels during the diurnal cycle. Two-week-old Arabidopsis plants were grown under short-day conditions (8 h of L/16 h of D), transferred to D for 24 h, and sampled at 4-h intervals. Expression levels were determined by quantitative RT-PCR with gene-specific primer sets.

(C) and (D) Relative levels of histone H3 modifications, K4me3 (C) and K9/14ac (D), of phyA during the diurnal cycle. The relative enrichment of histone H3 modifications on region 3 of the phyA locus was determined by ChIP assays using specific antibodies.

Error bars indicate sd (n = 3).