Figure 7.

Comparison of DNA Methylation Status of the phyA Locus in the D/L Transition.

(A) Schematic diagram of partial phyA gene structure showing the promoter (P), 5′ UTR (5′U), and exon 1 (e1). Black bars below the map represent regions analyzed by bisulfite sequencing. Each fragment was amplified using bisulfite-modified genomic DNA and inserted into pCR2.1-TOPO vector. P1 and P1, Promoter regions; U1 and U2, 5′ UTRs; E1 and E2, exon 1 regions.

(B) Analysis of average cytosine methylation (CG, CHG, and CHH) in the D/L transition. Sequences of 10 to 25 clones for each region were analyzed, and the results are shown in Supplemental Figure 9 online. H = A, T, or C.