Skip to main content
. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Immunol Rev. 2011 May;241(1):180–205. doi: 10.1111/j.1600-065X.2011.01011.x

Fig. 2. Preferential localization of liCTLA-4 in lipid rafts.

Fig. 2

2×108 T cells were activated for 3 days by plate-bound anti-CD3 and anti-CD28 mAbs + IL-2 and lysed. Lysates were subjected to sucrose gradients to separate lipid rafts and membrane fractions. The full length and li forms were immunoprecipitated using anti-CTLA-4 N-terminal (4F10) or anti-CTLA-4 C-terminal (polyclonal) Abs and detected by c-terminal polyclonal Abs by western blot analysis.