Figure 4.

In vivo CAI-1 isotope labeling experiments. In vivo CAI-1 labeling experiments were performed by supplementing l-methionine or d₈-l-methionine in combination with glucose or ¹³C-glucose in M9 minimal medium. HRMS spectra are enlarged to show specific regions around molecular weights 215, 218, 225, and 228. Corresponding chemical structures, compound formulas, and expected molecular weights are indicated at the bottom of each column. In the structures, predicted deuterium atoms and ¹³C carbon atoms are denoted with “D” and “∗”, respectively.