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. 2011 Feb 14;46(3):193–204. doi: 10.1530/JME-10-0117

Figure 1.

Figure 1

Basal and cytokine-stimulated production of nitric oxide in islets. Expression of (A) iNOS (Nos2) mRNA and (B) iNOS protein in UCP2+/+ and UCP2−/− islets. 0=untreated control islets, C2=IL1β+IFNγ and C3=IL1β+IFNγ+TNF-α. Data were normalized to β-actin expression, as shown in the representative blot (inset). The effect of CAPE (CP) in the presence of three cytokines is also shown. N=3 or greater. (C) Nitric oxide (as nitrite normalized to total protein) was measured in supernatant from cultures of islets after 24 h in the absence or presence of iNOS inhibitor 1400W (10 μmol/l) or CAPE (10 μmol/l). N=4–8. *P<0·05 compared with respective basal group; star indicates P<0·05 compared with UCP2+/+ basal; #P<0·05 compared with respective cytokine-treated group.