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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Nat Immunol. 2011 Feb 27;12(4):295–303. doi: 10.1038/ni.2005

Figure 7.

Figure 7

Control of transcription factor induction by mTORC1 and mTORC2. (a) Lineage-specific transcription factor mRNA expression in wild-type, T-Rheb−/− and T-Rictor−/− CD4+ T cells skewed to TH1, TH2, or TH17 as before. ND = not detected. Units are fold over 18S rRNA (for Tbx21 and Gata3) or ddCT arbitrary units (for Rorc). (b) Flow cytometric analysis of wild-type, T-Rheb−/− and T-Rictor−/− CD4+ T cells skewed to TH1, TH2, or TH17 and stained for T-bet, GATA-3, and RORγT. (c) Flow cytometric analysis of cells nucleofected as in 6g and skewed to TH1 and TH2. The shaded histogram represents wild-type TH2 cells (for T-bet) and wild-type TH1 cells (for GATA-3). (d) Flow cytometric analysis of naive 5C.C7 T cells skewed to TH1 or TH2 in the presence or absence of VLD rapamycin. All data are representative of at least three independent experiments, except for c, which was performed twice.