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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Microcirculation. 2008 May;15(4):359–371. doi: 10.1080/10739680701762656

FIGURE 1.

FIGURE 1

Expression of TRP channels in bEnd3 cells and mouse brain microvessels. Total RNA was reverse transcribed with primers specific to the TRPC, TRPV and TRPM isoforms. Both bEnd3 cells and freshly isolated mouse brain microvessels express message for TRPC1, C2, C4 and C7, while mouse brain microvessels also express message for TRPC3, C5 and C6. TRPV2 and TRPV4 are expressed in both tissues, with message for TRPV6 also seen in the mouse brain microvessels. Finally, both source tissues express TRPM2, M3, M4 and M7 only. These results do not reflect relative expression levels of each transcript. Gels shown are representative blots for 3–4 separate experiments.