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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Microcirculation. 2008 May;15(4):359–371. doi: 10.1080/10739680701762656

FIGURE 3.

FIGURE 3

bEnd3 cell calcium responses to increasing temperature. bEnd3 cells were plated on glass coverslips and loaded with Fura-2/AM as described. Coverslips were placed in a room-temperature imaging system and fluorescent imaging was started. After 2–3 min, the heat in the imaging chamber was turned on (arrow), and [Ca]i was assessed as the temperature increased. bEnd3 cell calcium levels remained steady until the imaging chamber reached 26–30°C (a representative experiment is shown, n=5). This is the temperature range that has been found to activate TRPV channels in heterologous expression systems, indicating the presence of functional endogenous TRPV channels.